Direct Reprogramming of Resident NG2 Glia into Neurons with Properties of Fast-Spiking Parvalbumin-Containing Interneurons.
Stem Cell Reports
; 9(3): 742-751, 2017 09 12.
Article
in En
| MEDLINE
| ID: mdl-28844658
ABSTRACT
Converting resident glia into functional and subtype-specific neurons in vivo by delivering reprogramming genes directly to the brain provides a step forward toward the possibility of treating brain injuries or diseases. To date, it has been possible to obtain GABAergic and glutamatergic neurons via in vivo conversion, but the precise phenotype of these cells has not yet been analyzed in detail. Here, we show that neurons reprogrammed using Ascl1, Lmx1a, and Nurr1 functionally mature and integrate into existing brain circuitry and that the majority of the reprogrammed neurons have properties of fast-spiking, parvalbumin-containing interneurons. When testing different combinations of genes for neural conversion with a focus on pro-neural genes and dopamine fate determinants, we found that functional neurons can be generated using different gene combinations and in different brain regions and that most of the reprogrammed neurons become interneurons, independently of the combination of reprogramming factors used.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parvalbumins
/
Action Potentials
/
Neuroglia
/
Cellular Reprogramming
/
Interneurons
Limits:
Animals
Language:
En
Journal:
Stem Cell Reports
Year:
2017
Document type:
Article
Affiliation country:
Suecia