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Otitis Media and Nasopharyngeal Colonization in ccl3-/- Mice.
Deniffel, Dominik; Nuyen, Brian; Pak, Kwang; Suzukawa, Keigo; Hung, Jun; Kurabi, Arwa; Wasserman, Stephen I; Ryan, Allen F.
Affiliation
  • Deniffel D; Department of Surgery/Otolaryngology, University of California, San Diego, La Jolla, California, USA.
  • Nuyen B; Ortenau Hospital Offenburg Department of Radiology, Offenburg, Germany.
  • Pak K; Department of Surgery/Otolaryngology, University of California, San Diego, La Jolla, California, USA.
  • Suzukawa K; Veterans Administration San Diego Healthcare System, San Diego, California, USA.
  • Hung J; Department of Surgery/Otolaryngology, University of California, San Diego, La Jolla, California, USA.
  • Kurabi A; Veterans Administration San Diego Healthcare System, San Diego, California, USA.
  • Wasserman SI; Department of Surgery/Otolaryngology, University of California, San Diego, La Jolla, California, USA.
  • Ryan AF; University of Tokyo Department of Otolaryngology, Tokyo, Japan.
Infect Immun ; 85(11)2017 11.
Article in En | MEDLINE | ID: mdl-28847849
ABSTRACT
We previously found CC chemokine ligand 3 (CCL3) to be a potent effector of inflammation during otitis media (OM) exogenous CCL3 rescues the OM phenotype of tumor necrosis factor-deficient mice and the function of macrophages deficient in several innate immune molecules. To further delineate the role of CCL3 in OM, we evaluated middle ear (ME) responses of ccl3-/-mice to nontypeable Haemophilus influenzae (NTHi). CCL chemokine gene expression was evaluated in wild-type (WT) mice during the complete course of acute OM. OM was induced in ccl3-/- and WT mice, and infection and inflammation were monitored for 21 days. Phagocytosis and killing of NTHi by macrophages were evaluated by an in vitro assay. The nasopharyngeal bacterial load was assessed in naive animals of both strains. Many CCL genes showed increased expression levels during acute OM, with CCL3 being the most upregulated, at levels 600-fold higher than the baseline. ccl3-/- deletion compromised ME bacterial clearance and prolonged mucosal hyperplasia. ME recruitment of leukocytes was delayed but persisted far longer than in WT mice. These events were linked to a decrease in the macrophage capacity for NTHi phagocytosis and increased nasopharyngeal bacterial loads in ccl3-/- mice. The generalized impairment in inflammatory cell recruitment was associated with compensatory changes in the expression profiles of CCL2, CCL7, and CCL12. CCL3 plays a significant role in the clearance of infection and resolution of inflammation and contributes to mucosal host defense of the nasopharyngeal niche, a reservoir for ME and upper respiratory infections. Therapies based on CCL3 could prove useful in treating or preventing persistent disease.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Otitis Media / Nasopharynx / Haemophilus influenzae / Ear, Middle / Chemokine CCL3 / Haemophilus Infections Type of study: Prognostic_studies Limits: Animals Language: En Journal: Infect Immun Year: 2017 Document type: Article Affiliation country: Estados Unidos

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Otitis Media / Nasopharynx / Haemophilus influenzae / Ear, Middle / Chemokine CCL3 / Haemophilus Infections Type of study: Prognostic_studies Limits: Animals Language: En Journal: Infect Immun Year: 2017 Document type: Article Affiliation country: Estados Unidos