Alpha-Glucan, Water Dikinase 1 Affects Starch Metabolism and Storage Root Growth in Cassava (Manihot esculenta Crantz).
Sci Rep
; 7(1): 9863, 2017 08 29.
Article
in En
| MEDLINE
| ID: mdl-28852191
ABSTRACT
ABSTARCT Regulation of storage root development by source strength remains largely unknown. The cassava storage root delay (srd) T-DNA mutant postpones storage root development but manifests normal foliage growth as wild-type plants. The SRD gene was identified as an orthologue of α-glucan, water dikinase 1 (GWD1), whose expression is regulated under conditions of light/dark cycles in leaves and is associated with storage root development. The GWD1-RNAi cassava plants showed both retarded plant and storage root growth, as a result of starch excess phenotypes with reduced photosynthetic capacity and decreased levels of soluble saccharides in their leaves. These leaves contained starch granules having greatly increased amylose content and type C semi-crystalline structures with increased short chains that suggested storage starch. In storage roots of GWD1-RNAi lines, maltose content was dramatically decreased and starches with much lower phosphorylation levels showed a drastically reduced ß-amylolytic rate. These results suggested that GWD1 regulates transient starch morphogenesis and storage root growth by decreasing photo-assimilation partitioning from the source to the sink and by starch mobilization in root crops.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Starch
/
Manihot
/
Phosphotransferases (Paired Acceptors)
/
Plant Roots
/
Carbohydrate Metabolism
/
Glucans
Language:
En
Journal:
Sci Rep
Year:
2017
Document type:
Article
Affiliation country:
China