Role of herpesviruses in chronic periodontitis and their association with clinical parameters and in increasing severity of the disease.

ABSTRACT

OBJECTIVE: This study aims to assess the role of herpesviruses in chronic periodontitis and their association with clinical parameters and in increasing severity. MATERIALS AND METHODS: This was a prospective case-control study. Ethical approval and prior consent were taken. A subgingival plaque sample was collected from a total of 300 patients and 300 controls and processed for DNA extraction and multiplex polymerase chain reaction for detection of herpesviruses. RESULTS: The most predominant age group affected was 41-50 followed by 31-40 years and male patients outnumbered the female patients. Herpes simplex virus (HSV)-1 (46.6%) was the most common Herpesvirus followed by HSV-2 (34.6%), Epstein-Barr viruses (EBV) (30.6%), and cytomegalovirus (CMV) (19.3%) in chronic periodontitis. Herpesviruses were significantly associated with increasing severity of the disease and had shown differences in their association with clinical parameters. Multiple herpesvirus infection was seen in patients with severe chronic periodontitis. The most common combination was HSV-1 + HSV-2 and HSV-1 + HSV-2 + EBV. CONCLUSIONS: HSV-1 was the most common herpesviruses implicated in the etiology of the chronic periodontitis followed by HSV-2, EBV and CMV. A herpesvirus differs in association with clinical parameters and plays an important role in increasing severity of the disease.