Scanning ion conductance microscopy for visualizing the three-dimensional surface topography of cells and tissues.
Semin Cell Dev Biol
; 73: 125-131, 2018 01.
Article
in En
| MEDLINE
| ID: mdl-28939037
ABSTRACT
Scanning ion conductance microscopy (SICM), which belongs to the family of scanning probe microscopy, regulates the tip-sample distance by monitoring the ion current through the use of an electrolyte-filled nanopipette as the probing tip. Thus, SICM enables "contact-free" imaging of cell surface topography in liquid conditions. In this paper, we applied hopping mode SICM for obtaining topographical images of convoluted tissue samples such as trachea and kidney in phosphate buffered saline. Some of the SICM images were compared with the images obtained by scanning electron microscopy (SEM) after drying the same samples. We showed that the imaging quality of hopping mode SICM was excellent enough for investigating the three-dimensional surface structure of the soft tissue samples. Thus, SICM is expected to be used for imaging a wide variety of cells and tissues - either fixed or alive- at high resolution under physiologically relevant liquid conditions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Trachea
/
Kidney Glomerulus
/
Microscopy
Limits:
Animals
Language:
En
Journal:
Semin Cell Dev Biol
Journal subject:
EMBRIOLOGIA
Year:
2018
Document type:
Article
Affiliation country:
Japón