An analysis of suppressing migratory effect on human urinary bladder cancer cell line by silencing of snail-1.
Biomed Pharmacother
; 96: 545-550, 2017 Dec.
Article
in En
| MEDLINE
| ID: mdl-29032338
ABSTRACT
BACKGROUND:
Snail-1 actively participates in tumor progression, invasion, and migration. Targeting snail-1 expression can suppress the EMT process in cancer. The aim of this study was to investigate the effect of snail1 silencing on urinary bladder cancer.METHODS:
Quantitative RT-PCR was used to detect snail-1 and other related metastatic genes expression following siRNA knockdown in urinary bladder cancer EJ-138 cells. The protein level of snail1 was assessed by Western blot. MTT and TUNEL assays were assessed to understand if snail-1 had survival effects on EJ-138 cells. Scratch wound healing assay measured cell motility effects after snail1 suppression.RESULTS:
The significant silencing of snail-1 reached 60pmol siRNA in a 48-h post-transfection. The result of scratch assay showed that snail-1 silencing significantly decreased Vimentin, MMPs, and CXCR4 expression; however, expression of E-cadherin was induced. The cell death assay indicated that snail-1 played the crucial role in bladder cancer survival rate.CONCLUSION:
These results propose that snail-1 plays a major role in the progression and migration of urinary bladder cancer, and can be a potential therapeutic target for target therapy of invasive urinary bladder cancer.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Urinary Bladder Neoplasms
/
Cell Movement
/
Gene Silencing
/
Snail Family Transcription Factors
Limits:
Humans
Language:
En
Journal:
Biomed Pharmacother
Year:
2017
Document type:
Article
Affiliation country:
Irán