Labeling and identifying cell-specific proteomes in the mouse brain.
Nat Biotechnol
; 36(2): 156-159, 2018 02.
Article
in En
| MEDLINE
| ID: mdl-29251727
ABSTRACT
We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain
/
Proteome
/
Amino Acyl-tRNA Synthetases
/
Neurons
Limits:
Animals
Language:
En
Journal:
Nat Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2018
Document type:
Article
Affiliation country:
Reino Unido