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In search of the best xenogeneic material for a paediatric conduit: an experimental study.
Zhuravleva, Irina Y; Nichay, Nataliya R; Kulyabin, Yuriy Y; Timchenko, Tatyana P; Korobeinikov, Alexander A; Polienko, Yuliya F; Shatskaya, Svetlana S; Kuznetsova, Elena V; Voitov, Alexey V; Bogachev-Prokophiev, Alexander V; Karaskov, Alexander M.
Affiliation
  • Zhuravleva IY; Laboratory of Biological Prostheses, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Nichay NR; Department of Congenital Heart Disease, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Kulyabin YY; Department of Congenital Heart Disease, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Timchenko TP; Laboratory of Biological Prostheses, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Korobeinikov AA; Laboratory of Biological Prostheses, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Polienko YF; Laboratory of Nitrogen Compounds, N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, SB RAS, Novosibirsk, Russian Federation.
  • Shatskaya SS; Laboratory of Intercalation and Mechanochemical Reactions, Institute of Solid State Chemistry and Mechanochemistry, SB RAS, Novosibirsk, Russian Federation.
  • Kuznetsova EV; Laboratory of Biological Prostheses, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Voitov AV; Department of Congenital Heart Disease, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Bogachev-Prokophiev AV; Heart Valve Surgery Department, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
  • Karaskov AM; Heart Valve Surgery Department, E. Meshalkin National Medical Research Center, Novosibirsk, Russian Federation.
Interact Cardiovasc Thorac Surg ; 26(5): 738-744, 2018 05 01.
Article in En | MEDLINE | ID: mdl-29346675
OBJECTIVES: The development of calcification-resistant bioprosthetic materials is a very important challenge for paediatric surgery. The subcutaneous implantation in rats is the well-known first-stage model for this kind of research. Using this model, we aimed to compare calcification of the porcine aortic wall and bovine pericardium and jugular vein wall cross-linked with glutaraldehyde (GA) and ethylene glycol diglycidyl ether (DE). We also determined the efficacy of DE-preserved tissue modification with 2-(2-carboxyethylamino)ethylidene-1,1-bisphosphonic acid (CEABA). METHODS: Three groups of each biomaterial were evaluated: GA-treated, DE-treated and DE + CEABA-treated. The microstructure of non-implanted biomaterials was assessed by light microscopy after Picro Mallory staining; the phosphorus content of the DE and DE + CEABA samples was assessed by atomic emission spectrometry. Samples were implanted subcutaneously into young rats for 10 and 60 days. The explant end-point included quantitative calcification assessment by atomic absorption spectrophotometry and light microscopy examination after von Kossa staining. RESULTS: All GA-treated biomaterials had a high calcium-binding capacity (>100 µg/mg dry tissue). DE preservation decreased the vein wall and pericardium calcium content by 4- and 40-fold, respectively, but was ineffective for the aortic wall. The calculated CEABA content was almost equal in the vein wall and pericardium (17.7 and 18.5 µM/g) and slightly less in the aortic wall (15 µM/g) (P = 0.011). CEABA effectively reduced mineralization in the DE aortic wall and DE pericardium to 10.1 (7.8-21.1) and 0.95 (0.57-1.38) µg/mg but had no effect in the DE vein wall. Mineralization in the GA- and DE-treated aortic and vein walls was predominantly associated with elastin. CEABA modification decreased elastin calcification but did not block it completely. CONCLUSIONS: Each xenogeneic material requires individual anticalcification strategy. DE + CEABA pretreatment demonstrates a high mineralization-blocking efficacy for the bovine pericardium and should be employed to further develop the paediatric pericardial conduit. Aortic wall calcification cannot be blocked completely using this strategy.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Aorta / Pericardium / Glutaral / Diphosphonates / Epoxy Resins / Vascular Calcification / Jugular Veins Limits: Animals Language: En Journal: Interact Cardiovasc Thorac Surg Journal subject: ANGIOLOGIA / CARDIOLOGIA Year: 2018 Document type: Article Country of publication: Reino Unido

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Aorta / Pericardium / Glutaral / Diphosphonates / Epoxy Resins / Vascular Calcification / Jugular Veins Limits: Animals Language: En Journal: Interact Cardiovasc Thorac Surg Journal subject: ANGIOLOGIA / CARDIOLOGIA Year: 2018 Document type: Article Country of publication: Reino Unido