Silencing of LncRNA TCONS_00088786 reduces renal fibrosis through miR-132.
Eur Rev Med Pharmacol Sci
; 22(1): 166-173, 2018 01.
Article
in En
| MEDLINE
| ID: mdl-29364484
ABSTRACT
OBJECTIVE:
To examine the role of long non-coding ribonucleic acid (LncRNA) TCONS_00088786 in the development of renal interstitial fibrosis and its potential mechanism in this process. MATERIALS ANDMETHODS:
Unilateral ureteral obstruction (UUO) was used to induce tubulointerstitial fibrosis. Masson staining showed the degree of renal fibrosis in UUO mice. Immunohistochemistry and immunofluorescence were performed to detect the fibrosis-related proteins, the 24-h urine volume and protein content. The renal functions were reflected via serum creatinine (Scr) and blood urea nitrogen (BUN). Changes in lncRNATCONS_00088786, miR-132 and collagen I and III in the development process of renal fibrosis were detected through reverse transcription-polymerase chain reaction (RT-PCR). Small interfering RNA (siRNA) was transfected into NRK52E cells to mimic the knockdown. Western blot was adopted to detect the changes in miR-132, collagen I and III after the siRNA was transfected by transforming growth factor-ß (TGF-ß) for 24 h.RESULTS:
With the development of renal fibrosis, lncRNA TCONS_00088786 and miR-132 were increased gradually. After the knockdown of lncRNA TCONS_00088786, miR-132 was decreased and fibrosis-related protein was also decreased.CONCLUSIONS:
Decreased lncRNA TCONS _00088786 inhibits renal interstitial fibrosis by reducing miR-132 and it may be a potential novel molecular target for the treatment of renal interstitial fibrosis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ureteral Obstruction
/
MicroRNAs
/
RNA, Long Noncoding
Limits:
Animals
Language:
En
Journal:
Eur Rev Med Pharmacol Sci
Journal subject:
FARMACOLOGIA
/
TOXICOLOGIA
Year:
2018
Document type:
Article
Affiliation country:
China