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Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy.
Mathias, Sven; Fischer, Simon; Handrick, René; Fieder, Jürgen; Schulz, Patrick; Bradl, Harald; Gorr, Ingo; Gamer, Martin; Otte, Kerstin.
Affiliation
  • Mathias S; University of Applied Sciences Biberach, Institute of Applied Biotechnology, Hubertus-Liebrecht-Straße 35, 88400 Biberach, Germany. Electronic address: mathias@hochschule-bc.de.
  • Fischer S; Boehringer Ingelheim Pharma GmbH & Co KG, Cell Culture Development CMB, Birkendorfer Straße 65, 88397 Biberach, Germany.
  • Handrick R; University of Applied Sciences Biberach, Institute of Applied Biotechnology, Hubertus-Liebrecht-Straße 35, 88400 Biberach, Germany.
  • Fieder J; Boehringer Ingelheim Pharma GmbH & Co KG, Early Stage Bioprocess Development, Birkendorfer Straße 65, 88397 Biberach, Germany.
  • Schulz P; Boehringer Ingelheim Pharma GmbH & Co KG, Cell Culture Development CMB, Birkendorfer Straße 65, 88397 Biberach, Germany.
  • Bradl H; Boehringer Ingelheim Pharma GmbH & Co KG, Cell Culture Development CMB, Birkendorfer Straße 65, 88397 Biberach, Germany.
  • Gorr I; Boehringer Ingelheim Pharma GmbH & Co KG, Early Stage Bioprocess Development, Birkendorfer Straße 65, 88397 Biberach, Germany.
  • Gamer M; Boehringer Ingelheim Pharma GmbH & Co KG, Early Stage Bioprocess Development, Birkendorfer Straße 65, 88397 Biberach, Germany.
  • Otte K; University of Applied Sciences Biberach, Institute of Applied Biotechnology, Hubertus-Liebrecht-Straße 35, 88400 Biberach, Germany.
J Biotechnol ; 271: 47-55, 2018 Apr 10.
Article in En | MEDLINE | ID: mdl-29477386
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Antibodies, Bispecific / Batch Cell Culture Techniques Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Biotechnol Journal subject: BIOTECNOLOGIA Year: 2018 Document type: Article Country of publication: Países Bajos
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Antibodies, Bispecific / Batch Cell Culture Techniques Type of study: Prognostic_studies Limits: Animals Language: En Journal: J Biotechnol Journal subject: BIOTECNOLOGIA Year: 2018 Document type: Article Country of publication: Países Bajos