Distinguishing between whole cells and cell debris using surface plasmon coupled emission.

ABSTRACT

Distinguishing between whole cells and cell debris is important in microscopy, e.g., in screening of pulmonary patients for infectious tuberculosis. We propose and theoretically demonstrate that whole cells and cell debris can be distinguished from the far-field pattern of surface plasmon coupled emission (SPCE) of a fluorescently-labeled sample placed on a thin metal layer. If fluorescently-labeled whole cells are placed on the metal film, SPCE takes place simultaneously at two or more different angles and creates two or more distinct rings in the far field. By contrast, if fluorescently-labeled cell debris are placed on the metal film, SPCE takes place at only one angle and creates one ring in the far-field. We find that the angular separation of the far-field rings is sufficiently distinct to use the presence of one or more rings to distinguish between whole cells and cell debris. The proposed technique has the potential for detection without the use of a microscope.