Molecular surveillance of canine distemper virus in diarrhoetic puppies in northeast China from May 2014 to April 2015.
J Vet Med Sci
; 80(6): 1029-1033, 2018 Jun 29.
Article
in En
| MEDLINE
| ID: mdl-29695673
ABSTRACT
To trace the prevalence of canine distemper virus (CDV) in diarrhoetic dogs, a total of 201 stool samples were collected in the Heilongjiang province of northeastern China from May 2014 to April 2015. The 201 fecal samples were subjected to the detection of CDV by using RT-PCR targeting the partial N gene, phylogenetic analysis based on the complete H gene, and co-infection analysis. Results indicated that 24.88% (50/201) of the samples were positive for CDV. The fifty CDV samples exhibited an overall co-infection rate of 94% (47/50) with four enteric viruses (82%, 41/50) and five bacteria (72%, 36/50). The positivity rate of CDV exhibited differences among regions, seasons, ages and immunization status. Phylogenetic analysis of the complete H genes (n=6) revealed that the CDV strains identified in our study belonged to the Asia-1 group, and showed genetic diversities. These data provide evidence that there are a number of genetically diverse CDV Asia-1 strains circulating in diarrhoetic dogs in northeastern China; the CDV-affected animals exhibit the high co-infection with other enteric viruses and bacteria.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Distemper
/
Distemper Virus, Canine
/
Dog Diseases
Type of study:
Risk_factors_studies
/
Screening_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
J Vet Med Sci
Journal subject:
MEDICINA VETERINARIA
Year:
2018
Document type:
Article