[Expression and significance of c-fos in resistant cell line TU177/VCR of larynx squamous cell carcinoma].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
; 53(4): 281-285, 2018 Apr 07.
Article
in Zh
| MEDLINE
| ID: mdl-29747253
ABSTRACT
Objective:
To explore the effect of c-fos on multidrug resistance of laryngeal cancer TU177 cells.Method:
Increasing drug concentration gradient is adopted to establish the stability of the laryngeal cancer drug resistance in cell line; RT-PCR and Western blot were used to detect difference of the c-fos between TU177 and TU177/VCR cells; plasmids with human c-fos knockdown or over expression were transfected into TU177/VCR and TU177 cells respectively, and the effects of different treatment on cell proliferation were investigated with MTT.Results:
The drug resistance of TU177/VCR cells was 26.25-fold in vincristine (VCR), 7.33-fold in Paclitaxel (TAX), 2.41 in cisplatin (DDP), and 5.50 in 5-fluorouracil (5-FU), comparing with TU177( P<0.05). The TU177/VCR cells had significantly higher c-fos expression compared to TU177 cells( P<0.05). The results showed that the IC(50) values of 5-FU for the NC group and c-fos shRNA group were (306.2±6.3)µmol/L and (81.3±3.9)µmol/L, respectively, which was decreased by 73% in the c-fos shRNA group compared to that in the NC group (P<0.05). Similarly, the results showed that the IC(50) values for 5-FU were (55.3±9.4) µmol/L in NC group and (288.1±7.3)µmol/L in c-fos WT group, which was increased 5.21-fold in c-fos WT cells.Conclusion:
C-fos plays important role in multidrug resistance of larynx cancer cell TU177/VCR, and might become a new molecular target for laryngeal cancer treatment.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinoma, Squamous Cell
/
Laryngeal Neoplasms
/
Proto-Oncogene Proteins c-fos
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
RNA, Small Interfering
/
Antineoplastic Agents
Limits:
Humans
Language:
Zh
Journal:
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
Year:
2018
Document type:
Article
Affiliation country:
China