Characterization and prebiotic activity in vitro of inulin-type fructan from Codonopsis pilosula roots.
Carbohydr Polym
; 193: 212-220, 2018 Aug 01.
Article
in En
| MEDLINE
| ID: mdl-29773375
ABSTRACT
The inulin-type fructan was obtained by DEAE anion exchange chromatography from C. pilosula Nannf. var. modesta (Nannf.) L. T. Shen, after optimized extract condition, which was established by response surface methodology, designed using Box-Behnken factorial design and the optimum condition were extracting 2.5â¯h with ratio of solvent to material 40â¯mL/g at 100⯰C, twice. The maximum extraction yield was 20.6⯱â¯0.2%. It was confirmed as ß-(2-1) linkage fructan, with terminal glucose, and with a degree of polymerization of 2-17 (DPavâ¯=â¯6), shown by the results of methanolysis, methylation, nuclear magnetic resonance and molecular weight determination. The prebiotic activity was proven on account of stimulation effect on Lactobacillus and pH reduction of medium in vitro. The results indicated that the inulin from C. pilosula could be used as a potential natural source of prebiotics.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Plant Roots
/
Codonopsis
/
Prebiotics
/
Fructans
/
Inulin
Language:
En
Journal:
Carbohydr Polym
Year:
2018
Document type:
Article