<sup>â¿¿</sup>Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples.

Bennike, Tue Bjerg; Kastaniegaard, Kenneth; Padurariu, Simona; Gaihede, Michael; Birkelund, Svend; Andersen, Vibeke; Stensballe, Allan

^â¿¿Comparing the proteome of snap frozen, RNAlater preserved, and formalin-fixed paraffin-embedded human tissue samples.

Bennike, Tue Bjerg; Kastaniegaard, Kenneth; Padurariu, Simona; Gaihede, Michael; Birkelund, Svend; Andersen, Vibeke; Stensballe, Allan.

Affiliation

Bennike TB; Research Unit for Molecular Diagnostic and Clinical Research, Hospital of Southern Jutland, Aabenraa, Denmark.
Kastaniegaard K; Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Padurariu S; Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Gaihede M; Department of Otolaryngology, Head and Neck Surgery, Aalborg University Hospital, Aalborg, Denmark.
Birkelund S; Department of Otolaryngology, Head and Neck Surgery, Aalborg University Hospital, Aalborg, Denmark.
Andersen V; Department of Clinical Medicine, Aalborg University, Aalborg, Denmark.
Stensballe A; Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.

EuPA Open Proteom ; 10: 9-18, 2016 Mar.

Article in En | MEDLINE | ID: mdl-29900094

Key words

CAN, acetonitrile; DF, directly-frozen; FA, formic acid; FASP, filter-aided sample preparation; FDR, false discovery rate; FFPE, formalin-fixed; Formalin-fixed; HLA-A class I, histocompatibility antigen A-23 alpha chain; HLA-DRB1 class II, histocompatibility antigen DRB1-4 beta chain; Human colon mucosa; LFQ, label-free quantification; Mass spectrometry; PCA, principle component analysis; PSM, peptide spectral match; PTM, post-translational modification; Paraffin-embedded; Preservation; Proteomics; RNAlater; SDC, sodium deoxycholate; SDS, sodium dodecyl sulfate; TEAB, triethylammonium bicarbonate; iFFPE, immediately formalin-fixed; s, standard deviation; sFFPE, stored for 30 min prior to formalin-fixed

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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: EuPA Open Proteom Year: 2016 Document type: Article Affiliation country: Dinamarca Country of publication: Países Bajos

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