Overexpression of retinoblastomabinding protein 4 contributes to the radiosensitivity of AGS gastric cancer cells via phosphoinositide3kinase/protein kinase B pathway suppression.
Mol Med Rep
; 18(2): 1571-1581, 2018 Aug.
Article
in En
| MEDLINE
| ID: mdl-29901205
ABSTRACT
In the present study, the effects and underlying mechanism of RbAp48 on the radiosensitivity of AGS gastric cancer cells was investigated. Cell proliferation was determined with an MTT assay. Flow cytometry was performed to evaluate the cell cycle and apoptosis. Reverse transcriptionquantitative polymerase chain reaction and western blot analysis were performed to detect mRNA and protein expression, respectively, including RbAp48, phosphoinositide 3kinase (PI3K) and protein kinase B (Akt). The results revealed that radiation enhanced the expression level of RbAp48 in AGS cells, and that RbAp48 combined with radiation reduced AGS cell proliferation. In addition, RbAp48 combined with radiation resulted in G2 phase arrest and induced apoptosis via regulation of the PI3K/Akt pathway. In conclusion, it was demonstrated that overexpression of RbAp48 enhanced the radiosensitivity of AGS gastric cancer cells via suppression of PI3K/Akt pathway activity, suggesting that RbAp48 may hold potential as a gene therapeutic strategy in the future, aiding in the treatment of gastric cancer.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Radiation Tolerance
/
Gene Expression Regulation, Neoplastic
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Phosphatidylinositol 3-Kinases
/
Proto-Oncogene Proteins c-akt
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Retinoblastoma-Binding Protein 4
/
Gastric Mucosa
Limits:
Humans
Language:
En
Journal:
Mol Med Rep
Year:
2018
Document type:
Article