Development of multiplex real-time reverse-transcriptase polymerase chain reaction assay for simultaneous detection of Zika, dengue, yellow fever, and chikungunya viruses in a single tube.
J Med Virol
; 90(11): 1681-1686, 2018 11.
Article
in En
| MEDLINE
| ID: mdl-29979812
ABSTRACT
Zika virus (ZIKV), dengue virus (DENV), chikungunya virus (CHIKV) and yellow fever virus (YFV) share the same mosquito vectors and have similar clinical manifestations early stage of infection. Therefore, simultaneously differentiating these viruses from each other is necessary. We developed a multiplex real-time reverse-transcriptase polymerase chain reaction (RT-PCR) assay for the differentiation of these four viruses in a single tube. The linear range was established by regression analysis, and the R2 value for each virus was ≥0.98, and the 95% lower limit of detection for each virus was as follows (copies/reaction) ZIKV-Asian, 9; ZIKV-Africa, 15; CHIKV, 11; DENV-1, 19; DENV-2, 13; DENV-3, 24; DENV-4, 36; and YFV, 17. Meanwhile, our multiplex real-time RT-PCR has a good consistency with the commercial singleplex assay. In summary, the developed assay can be effectively used for the diagnosis of ZIKV, DENV, CHIKV, and YFV infections.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Yellow Fever
/
Reverse Transcriptase Polymerase Chain Reaction
/
Dengue
/
Multiplex Polymerase Chain Reaction
/
Real-Time Polymerase Chain Reaction
/
Chikungunya Fever
/
Zika Virus Infection
Type of study:
Diagnostic_studies
Limits:
Humans
Country/Region as subject:
Africa
Language:
En
Journal:
J Med Virol
Year:
2018
Document type:
Article
Affiliation country:
China