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Design of DNA rolling-circle templates with controlled fork topology to study mechanisms of DNA replication.
Monachino, Enrico; Ghodke, Harshad; Spinks, Richard R; Hoatson, Ben S; Jergic, Slobodan; Xu, Zhi-Qiang; Dixon, Nicholas E; van Oijen, Antoine M.
Affiliation
  • Monachino E; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia; Zernike Institute for Advanced Materials, University of Groningen, 9747 AG, Groningen, The Netherlands.
  • Ghodke H; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia.
  • Spinks RR; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia.
  • Hoatson BS; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia.
  • Jergic S; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia.
  • Xu ZQ; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia.
  • Dixon NE; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia.
  • van Oijen AM; Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, New South Wales, 2522, Australia. Electronic address: vanoijen@uow.edu.au.
Anal Biochem ; 557: 42-45, 2018 09 15.
Article in En | MEDLINE | ID: mdl-30016625
ABSTRACT
Rolling-circle DNA amplification is a powerful tool employed in biotechnology to produce large from small amounts of DNA. This mode of DNA replication proceeds via a DNA topology that resembles a replication fork, thus also providing experimental access to the molecular mechanisms of DNA replication. However, conventional templates do not allow controlled access to multiple fork topologies, which is an important factor in mechanistic studies. Here we present the design and production of a rolling-circle substrate with a tunable length of both the gap and the overhang, and we show its application to the bacterial DNA-replication reaction.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / DNA, Circular / Nucleic Acid Amplification Techniques / DNA Replication / Escherichia coli Language: En Journal: Anal Biochem Year: 2018 Document type: Article Affiliation country: Países Bajos
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Bacterial / DNA, Circular / Nucleic Acid Amplification Techniques / DNA Replication / Escherichia coli Language: En Journal: Anal Biochem Year: 2018 Document type: Article Affiliation country: Países Bajos