Concentrations of vanadium in urine and seminal plasma in relation to semen quality parameters, spermatozoa DNA damage and serum hormone levels.

ABSTRACT

Widespread human exposure to vanadium has been well documented. Vanadium exposure was reported to induce male reproductive toxicity in toxicological studies, yet human epidemiologic studies are lacking. Here we determined the associations between environmental exposure to vanadium and semen quality, spermatozoa DNA damage and serum reproductive hormones. Concentrations of vanadium in seminal plasma and repeated urine samples were determined among 764 men recruited from a reproductive medicine centre. Associations of vanadium concentrations with semen quality parameters (n = 764), DNA integrity measures (n = 404) and serum reproductive hormones (n = 381) were assessed by logistic or linear regression models with adjustment for potential confounders. Significant positive dose-response relationships were observed between vanadium concentrations in seminal plasma and tail length and serum estradiol, as well as odds ratios for a below-reference-value sperm concentration; whereas inverse relationships between seminal plasma vanadium with total testosterone (T) and free T (all p values for trends <0.05) were observed. These relationships were maintained after adjusting for seminal plasma concentrations of other elements (i.e., arsenic, cadmium, copper, selenium, or tin). No significant associations was revealed between urinary vanadium concentrations and semen quality, spermatozoa DNA integrity and reproductive hormones. Our findings suggested that elevated vanadium exposure may be adversely associated with male reproductive health, and that seminal plasma vanadium may be a more direct exposure biomarker for the male reproductive system than urinary vanadium.