Your browser doesn't support javascript.
loading
A high-throughput pipeline for validation of antibodies.
Sikorski, Krzysztof; Mehta, Adi; Inngjerdingen, Marit; Thakor, Flourina; Kling, Simon; Kalina, Tomas; Nyman, Tuula A; Stensland, Maria Ekman; Zhou, Wei; de Souza, Gustavo A; Holden, Lars; Stuchly, Jan; Templin, Markus; Lund-Johansen, Fridtjof.
Affiliation
  • Sikorski K; Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
  • Mehta A; K.G. Jebsen Center for Cancer Immunotherapy, University of Oslo, Oslo, Norway.
  • Inngjerdingen M; Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
  • Thakor F; Department of Pathology, Oslo University Hospital, Oslo, Norway.
  • Kling S; K.G. Jebsen Inflammation Research Centre, University of Oslo, Oslo, Norway.
  • Kalina T; Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
  • Nyman TA; Department of Pathology, Oslo University Hospital, Oslo, Norway.
  • Stensland ME; K.G. Jebsen Inflammation Research Centre, University of Oslo, Oslo, Norway.
  • Zhou W; NMI Natural and Medical Sciences Institute, University of Tübingen, Reutlingen, Germany.
  • de Souza GA; CLIP-Childhood Leukemia Investigation Prague, Department of Pediatric Hematology and Oncology, 2nd Faculty of Medicine, Charles University Prague and University Hospital Motol, Prague, Czech Republic.
  • Holden L; Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
  • Stuchly J; Department of Immunology, University of Oslo and Oslo University Hospital, Oslo, Norway.
  • Templin M; SeekQuence, Mountain View, CA, USA.
  • Lund-Johansen F; The Brain Institute, Universidade Federal do Rio Grande do Norte, Natal, Brazil.
Nat Methods ; 15(11): 909-912, 2018 11.
Article in En | MEDLINE | ID: mdl-30377371
ABSTRACT
Western blotting (WB) is widely used to test antibody specificity, but the assay has low throughput and precision. Here we used preparative gel electrophoresis to develop a capture format for WB. Fractions with soluble, size-separated proteins facilitated parallel readout with antibody arrays, shotgun mass spectrometry (MS) and immunoprecipitation followed by MS (IP-MS). This pipeline provided the means for large-scale implementation of antibody validation concepts proposed by an international working group on antibody validation (IWGAV).
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteomics / High-Throughput Screening Assays / Antibodies / Neoplasm Proteins / Neoplasms Limits: Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2018 Document type: Article Affiliation country: Noruega
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Proteomics / High-Throughput Screening Assays / Antibodies / Neoplasm Proteins / Neoplasms Limits: Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2018 Document type: Article Affiliation country: Noruega