Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization.
IUCrJ
; 5(Pt 6): 667-672, 2018 Nov 01.
Article
in En
| MEDLINE
| ID: mdl-30443351
ABSTRACT
This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
IUCrJ
Year:
2018
Document type:
Article
Affiliation country:
Alemania