Nano-encapsulation using macrocyclic carbohydrate polymers (ß-cyclodextrins) of Periploca angustifolia extract: Physical stability and protective effect against cadmium-induced alterations in HepG2 cells.
Int J Biol Macromol
; 125: 711-720, 2019 Mar 15.
Article
in En
| MEDLINE
| ID: mdl-30521926
ABSTRACT
The nano-encapsulation of Periploca angustifolia phenolic extract using the macrocyclic carbohydrate polymers (ßcyclodextrins) is a most approach compared with other encapsulation methods. In this work, the ßCyclodextrins-PAE complex stability has been evaluated by advanced analytical methods and techniques including HPLC, FTIR and XRD. The results showed that CdCl2 treatment caused a significant decrease in cell viability. The CdCl2-induced damage in the HepG2 cells were significantly ameliorated (pâ¯<â¯0.001) by treatment of the PAE and ßCyclodextrins-PAE complex. Thus, pretreatment with 100⯵gâ¯mL-1 of ßCyclodextrins-PAE complex significantly protect HepG2 cells against cytotoxicity induced by cadmium exposure more effectively than PAE only. However, Cd-intoxication significantly (pâ¯<â¯0.001) increased these enzymes activity. Additionally, reactive oxygen species generation was significantly decreased when cells were treated with nano-encapsulation PAE. The levels of supernatant antioxidant parameters including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and GSH were significantly (pâ¯<â¯0.001) decreased in Cd-treated cells with concomitant enhancement of lipid peroxidation. In addition, ßCyclodextrins-PAE pretreatment significantly (pâ¯<â¯0.01) inhibited Cd-exposure activated the apoptotic pathway caspace-3 and caspace-9. This effect may be due to the ability of ßCyclodextrins molecules to enhance stability and permeability properties.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cadmium
/
Plant Extracts
/
Protective Agents
/
Periploca
/
Macrocyclic Compounds
/
Beta-Cyclodextrins
/
Nanoparticles
Limits:
Humans
Language:
En
Journal:
Int J Biol Macromol
Year:
2019
Document type:
Article