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International Space Station conditions alter genomics, proteomics, and metabolomics in Aspergillus nidulans.
Romsdahl, Jillian; Blachowicz, Adriana; Chiang, Abby J; Chiang, Yi-Ming; Masonjones, Sawyer; Yaegashi, Junko; Countryman, Stefanie; Karouia, Fathi; Kalkum, Markus; Stajich, Jason E; Venkateswaran, Kasthuri; Wang, Clay C C.
Affiliation
  • Romsdahl J; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Rm 406A, Los Angeles, CA, 90089-9121, USA.
  • Blachowicz A; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Rm 406A, Los Angeles, CA, 90089-9121, USA.
  • Chiang AJ; Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, Pasadena, CA, 91101, USA.
  • Chiang YM; Department of Molecular Imaging and Therapy, Beckman Research Institute of City of Hope, 1500 East Duarte Road, Duarte, CA, 91010, USA.
  • Masonjones S; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Rm 406A, Los Angeles, CA, 90089-9121, USA.
  • Yaegashi J; Department of Microbiology & Plant Pathology and Institute of Integrative Genome Biology, University of California-Riverside, 900 University Avenue, Riverside, CA, 92521, USA.
  • Countryman S; Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, 1985 Zonal Avenue, Rm 406A, Los Angeles, CA, 90089-9121, USA.
  • Karouia F; BioServe Space Technologies, University of Colorado, 429 UCB, Boulder, CO, 80309, USA.
  • Kalkum M; Space Biosciences Division, NASA Ames Research Center, MS-239-4, Moffett Field, Mountain View, CA, 94035, USA.
  • Stajich JE; Department of Pharmaceutical Chemistry, School of Pharmacy, University of California San Francisco, 600 16th Street, San Francisco, CA, 94143, USA.
  • Venkateswaran K; Department of Molecular Imaging and Therapy, Beckman Research Institute of City of Hope, 1500 East Duarte Road, Duarte, CA, 91010, USA.
  • Wang CCC; Department of Microbiology & Plant Pathology and Institute of Integrative Genome Biology, University of California-Riverside, 900 University Avenue, Riverside, CA, 92521, USA.
Appl Microbiol Biotechnol ; 103(3): 1363-1377, 2019 Feb.
Article in En | MEDLINE | ID: mdl-30539259
ABSTRACT
The first global genomic, proteomic, and secondary metabolomic characterization of the filamentous fungus Aspergillus nidulans following growth onboard the International Space Station (ISS) is reported. The investigation included the A. nidulans wild-type and three mutant strains, two of which were genetically engineered to enhance secondary metabolite production. Whole genome sequencing revealed that ISS conditions altered the A. nidulans genome in specific regions. In strain CW12001, which features overexpression of the secondary metabolite global regulator laeA, ISS conditions induced the loss of the laeA stop codon. Differential expression of proteins involved in stress response, carbohydrate metabolic processes, and secondary metabolite biosynthesis was also observed. ISS conditions significantly decreased prenyl xanthone production in the wild-type strain and increased asperthecin production in LO1362 and CW12001, which are deficient in a major DNA repair mechanism. These data provide valuable insights into the adaptation mechanism of A. nidulans to spacecraft environments.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stress, Physiological / Gene Expression Regulation, Fungal / Carbohydrate Metabolism / Secondary Metabolism / Genes, Fungal Language: En Journal: Appl Microbiol Biotechnol Year: 2019 Document type: Article Affiliation country: Estados Unidos
Fulltext
Add to My VHL
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stress, Physiological / Gene Expression Regulation, Fungal / Carbohydrate Metabolism / Secondary Metabolism / Genes, Fungal Language: En Journal: Appl Microbiol Biotechnol Year: 2019 Document type: Article Affiliation country: Estados Unidos