A coumarin-based dual optical probe for homocysteine with rapid response time, high sensitivity and selectivity.
Talanta
; 196: 243-248, 2019 May 01.
Article
in En
| MEDLINE
| ID: mdl-30683359
ABSTRACT
In this study, a new coumarin-based fluorescent and chromogenic dual channel probe (DC) was used for the selective detection of homocysteine (Hcy) over other amino acids, especially for cysteine (Cys) and glutathione (GSH). When Hcy is present in the solution, the remarkable fluorescence enhancement and obvious blue shift in UV-vis spectra can be observed. In addition, the color change from purple to yellow can be observed clearly by unaided eyes. This probe DC has fast response time, excellent sensitivity and selectivity to Hcy. A linear relationship exists between the ratio of emissions at 486 and 625â¯nm, and Hcy can be detected in a wide concentration range (0-200⯵M). The signal-to-background ratio of fluorescence at 486â¯nm can reach 8.4, and the detection limit is calculated to be 3.5⯵M. The response mechanism is proved to be the Michael addition reaction by Hcy. Preliminary results on cell imaging enable the practical application of Hcy tracing in living cells.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Type of study:
Diagnostic_studies
Language:
En
Journal:
Talanta
Year:
2019
Document type:
Article