Serum-Free Organ Culture of the Embryonic Mouse Ureter.
Methods Mol Biol
; 1926: 31-38, 2019.
Article
in En
| MEDLINE
| ID: mdl-30742260
ABSTRACT
The ability to explant and then maintain embryonic tissues in organ culture makes it feasible to study the growth and differentiation of whole organs, or parts or combinations of them, in three dimensions. Moreover, the possible effects of biochemical manipulations or mutations can be explored by visualizing a growing organ. The mammalian renal tract comprises the kidney, ureter, and urinary bladder, and the focus of this chapter is organ culture of the embryonic mouse ureter in serum-free defined medium. Over the culture period, rudiments grow in length, smooth muscle differentiates, and the ureters then undergo peristalsis in a proximal to distal direction.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Organ Culture Techniques
/
Ureter
/
Urinary Bladder
/
Kidney
Limits:
Animals
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Document type:
Article
Affiliation country:
Reino Unido