Method to quantify cytokines and chemokines in mouse brain tissue using Bio-Plex multiplex immunoassays.
Methods
; 158: 22-26, 2019 04 01.
Article
in En
| MEDLINE
| ID: mdl-30742997
ABSTRACT
This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biological Assay
/
Cytokines
/
Malaria, Cerebral
/
Chemokines
/
High-Throughput Screening Assays
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods
Journal subject:
BIOQUIMICA
Year:
2019
Document type:
Article
Affiliation country:
Estados Unidos