Your browser doesn't support javascript.
loading
The Complete Mitochondrial Genome Sequences of the Philomycus bilineatus (Stylommatophora: Philomycidae) and Phylogenetic Analysis.
Yang, Tiezhu; Xu, Guolyu; Gu, Bingning; Shi, Yanmei; Mzuka, Hellen Lucas; Shen, Heding.
Affiliation
  • Yang T; National Demonstration Center for Experimental Fisheries Science Education, Shanghai OceanUniversity, China. yangtiezhu1234@163.com.
  • Xu G; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai OceanUniversity, Ministry of Education, China. yangtiezhu1234@163.com.
  • Gu B; Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution,Shanghai 201306, China. yangtiezhu1234@163.com.
  • Shi Y; National Demonstration Center for Experimental Fisheries Science Education, Shanghai OceanUniversity, China. xuguolv@126.com.
  • Mzuka HL; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Shanghai OceanUniversity, Ministry of Education, China. xuguolv@126.com.
  • Shen H; Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution,Shanghai 201306, China. xuguolv@126.com.
Genes (Basel) ; 10(3)2019 03 05.
Article in En | MEDLINE | ID: mdl-30841657
ABSTRACT
The mitochondrial genome (mitogenome) can provide information for phylogenetic analyses and evolutionary biology. We first sequenced, annotated, and characterized the mitogenome of Philomycus bilineatus in this study. The complete mitogenome was 14,347 bp in length, containing 13 protein-coding genes (PCGs), 23 transfer RNA genes, two ribosomal RNA genes, and two non-coding regions (A + T-rich region). There were 15 overlap locations and 18 intergenic spacer regions found throughout the mitogenome of P. bilineatus. The A + T content in the mitogenome was 72.11%. All PCGs used a standard ATN as a start codon, with the exception of cytochrome c oxidase 1 (cox1) and ATP synthase F0 subunit 8 (atp8) with TTG and GTG. Additionally, TAA or TAG was identified as the typical stop codon. All transfer RNA (tRNA) genes had a typical clover-leaf structure, except for trnS1 (AGC), trnS2 (TCA), and trnK (TTT). A phylogenetic analysis with another 37 species of gastropods was performed using Bayesian inference, based on the amino acid sequences of 13 mitochondrial PCGs. The results indicated that P. bilineatus shares a close ancestry with Meghimatium bilineatum. It seems more appropriate to reclassify it as Arionoidea rather than Limacoidea, as previously thought. Our research may provide a new meaningful insight into the evolution of P. bilineatus.
Subject(s)
Key words

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gastropoda / Whole Genome Sequencing / Mitochondria Limits: Animals Language: En Journal: Genes (Basel) Year: 2019 Document type: Article Affiliation country: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Gastropoda / Whole Genome Sequencing / Mitochondria Limits: Animals Language: En Journal: Genes (Basel) Year: 2019 Document type: Article Affiliation country: China