Forms of diagnostic material as sources of miRNA biomarkers in hepatocellular carcinoma: a preliminary study.
Biomark Med
; 13(7): 523-534, 2019 05 01.
Article
in En
| MEDLINE
| ID: mdl-30854869
ABSTRACT
Aim:
To assess the diagnostic value of selected miRNAs from various material collected from hepatocellular carcinoma (HCC) patients. Patients &methods:
Tissue, serum, urine and fecal samples from HCC patients and healthy individuals were screened for associated miRNAs using microarray analysis; the selected miRNAs were then validated by real time-quantitative PCR on 65 patients.Results:
Serum miR-122, a combination of serum miR-155 with miR-885-5p, a combination of urinary miR-532-3p with miR-765, and fecal miR-320a displayed 100% efficiency in discriminating patients from controls. A combination of urinary miR-532-3p and miR-765 allowed patients with neoplastic grade G3 to be distinguished from those with G1 and G2.Conclusion:
Additionally to serum, urine and feces also appeared to be valuable source of potential HCC noninvasive miRNA biomarkers.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Carcinoma, Hepatocellular
/
MicroRNAs
/
Liver Neoplasms
Type of study:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Limits:
Aged
/
Female
/
Humans
/
Male
Language:
En
Journal:
Biomark Med
Journal subject:
BIOQUIMICA
/
MEDICINA
Year:
2019
Document type:
Article
Affiliation country:
Polonia