Optimization of the alkaline comet assay for easy repair capacity quantification of oxidative DNA damage in PBMC from human volunteers using aphidicolin block.

Odongo, Grace A; Skatchkov, Ivan; Herz, Corinna; Lamy, Evelyn

Odongo, Grace A; Skatchkov, Ivan; Herz, Corinna; Lamy, Evelyn.

Affiliation

Odongo GA; Molecular Preventive Medicine, University Medical Center and Faculty of Medicine, University of Freiburg, Breisacher Straße 115b, 79106 Freiburg im Breisgau, Germany; Institute of Food Chemistry, Hamburg School of Food Science, University of Hamburg, Grindelallee 117, 20146 Hamburg, Germany.
Skatchkov I; Molecular Preventive Medicine, University Medical Center and Faculty of Medicine, University of Freiburg, Breisacher Straße 115b, 79106 Freiburg im Breisgau, Germany.
Herz C; Molecular Preventive Medicine, University Medical Center and Faculty of Medicine, University of Freiburg, Breisacher Straße 115b, 79106 Freiburg im Breisgau, Germany.
Lamy E; Molecular Preventive Medicine, University Medical Center and Faculty of Medicine, University of Freiburg, Breisacher Straße 115b, 79106 Freiburg im Breisgau, Germany. Electronic address: evelyn.lamy@uniklinik-freiburg.de.

DNA Repair (Amst) ; 77: 58-64, 2019 05.

Article in En | MEDLINE | ID: mdl-30889507

Subject(s)
Key words

Aphidicolin block; Brassica; Comet assay; DNA repair; Fresh and cryopreserved PBMC; Oxidative damage

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Damage / Leukocytes, Mononuclear / Aphidicolin / Oxidative Stress / Comet Assay / DNA Repair Type of study: Guideline Limits: Adult / Female / Humans / Male Language: En Journal: DNA Repair (Amst) Journal subject: BIOLOGIA MOLECULAR / BIOQUIMICA Year: 2019 Document type: Article Affiliation country: Alemania Country of publication: Países Bajos

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