Expression analysis of proteasome maturation protein (POMP) gene in Liaoning Cashmere goat.
Anim Biotechnol
; 31(4): 324-334, 2020 Aug.
Article
in En
| MEDLINE
| ID: mdl-30957645
ABSTRACT
Liaoning Cashmere goat is a precious genetic resource of China. To explore the relationship between POMP and cashmere growth, we analyzed the expression of POMP. POMP encodes a hudrophilic protein which is most closely related to bos. RT-PCR showed that POMP was expressed in skin, heart, liver, spleen, lung, and kidney tissues. Real-time PCR showed that the expression of POMP was more active in the secondary hair follicles than the primary hair follicles in anagen. In situ hybridization showed that POMP was obviously expressed in the Inner Root Sheath (IRS) but no expression in Outer Root. The treatment of fibroblasts with melatonin (MT), fibroblast growth factors 5 (FGF5) and insulin-like growth factors 1 (IGF-1) showed that MT/FGF5/IGF-1 much performance for inhibiting the expression of POMP; MT + FGF5 inhibited the expression of POMP; MT + IGF-1 promoted the expression of POMP. When Noggin expression is decreased by siRNA, the expression of POMP is inhibited. To sum up, POMP strongly expressed in the root sheath of hair follicles, related to the development of the primary and secondary hair follicle; In addition, by adding MT/FGF5/IGF-1 or interfering with the Noggin expression to regulate the expression of POMP, to control the growth of Liaoning Cashmere goat cashmere.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Goats
/
Molecular Chaperones
/
Hair Follicle
Limits:
Animals
Language:
En
Journal:
Anim Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2020
Document type:
Article
Affiliation country:
China