Quantitative RNA Analysis Using RNA-Seq.
Methods Mol Biol
; 1971: 95-108, 2019.
Article
in En
| MEDLINE
| ID: mdl-30980299
ABSTRACT
High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital readout of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe two different RNA-seq approaches, including one that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5' end of all Leishmania (and other trypanosomatid) mRNAs.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Messenger
/
Exons
/
RNA, Protozoan
/
Sequence Analysis, RNA
/
High-Throughput Nucleotide Sequencing
/
Leishmania
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Document type:
Article
Affiliation country:
Estados Unidos