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Quantitative RNA Analysis Using RNA-Seq.
Myler, Peter J; McDonald, Jacqueline A; Alcolea, Pedro J; Sur, Aakash.
Affiliation
  • Myler PJ; Center for Global Infectious Disease Research, Seattle Childrens Research Institute, 307 Westlake Ave N, Suite 500, Seattle, 98109-5219, WA, USA. peter.myler@seattlechildrens.org.
  • McDonald JA; Department of Global Health, University of Washington, Seattle, 98195, WA, USA. peter.myler@seattlechildrens.org.
  • Alcolea PJ; Department of Biomedical Informatics and Medical Education, University of Washington, Seattle, 98195, WA, USA. peter.myler@seattlechildrens.org.
  • Sur A; Center for Global Infectious Disease Research, Seattle Childrens Research Institute, 307 Westlake Ave N, Suite 500, Seattle, 98109-5219, WA, USA.
Methods Mol Biol ; 1971: 95-108, 2019.
Article in En | MEDLINE | ID: mdl-30980299
ABSTRACT
High-throughput sequencing of cDNA copies of mRNA (RNA-seq) provides a digital readout of mRNA levels over several orders of magnitude, as well as mapping the transcripts to the nucleotide level. Here we describe two different RNA-seq approaches, including one that exploits the 39-nucleotide mini-exon or spliced leader (SL) sequence found at the 5' end of all Leishmania (and other trypanosomatid) mRNAs.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Exons / RNA, Protozoan / Sequence Analysis, RNA / High-Throughput Nucleotide Sequencing / Leishmania Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: Estados Unidos
Fulltext
Add to My VHL
Print
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RNA, Messenger / Exons / RNA, Protozoan / Sequence Analysis, RNA / High-Throughput Nucleotide Sequencing / Leishmania Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2019 Document type: Article Affiliation country: Estados Unidos