Increased luminescence of the GloSensor cAMP assay in LßT2 cells does not correlate with cAMP accumulation under low pH conditions.
J Reprod Dev
; 65(4): 381-388, 2019 Aug 09.
Article
in En
| MEDLINE
| ID: mdl-31006726
ABSTRACT
Cyclic adenosine monophosphate (cAMP) plays a pivotal role in gonadotrope responses in the pituitary. Gonadotropin-releasing hormone (GnRH) mediated synthesis and secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) are regulated by both the Gs/cAMP and Gq/Ca2+ signaling pathways. Pituitary adenylate cyclase-activating polypeptide (PACAP) also regulates GnRH responsiveness in gonadotropes through the PACAP receptor, which activates the Gs/cAMP signaling pathway. Therefore, measuring intracellular cAMP levels is important for elucidating the molecular mechanisms of FSH and LH synthesis and secretion in gonadotropes. The GloSensor cAMP assay is useful for detecting cAMP levels in intact, living cells. In this study, we found that increased GloSensor luminescence intensity did not correlate with cAMP accumulation in LßT2 cells under low pH conditions. This result indicates that cell type and condition must be considered when using GloSensor cAMP.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Biological Assay
/
Cyclic AMP
/
Gonadotrophs
/
Luminescent Measurements
Type of study:
Evaluation_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
J Reprod Dev
Journal subject:
MEDICINA REPRODUTIVA
Year:
2019
Document type:
Article
Affiliation country:
Japón
Publication country:
JAPAN
/
JAPON
/
JAPÃO
/
JP