Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry.
Methods Mol Biol
; 1989: 139-146, 2019.
Article
in En
| MEDLINE
| ID: mdl-31077104
ABSTRACT
Mass cytometry is a multi-parametric technique that offers insight into functional and biological systems at a single cell level (Tanner et al., Cancer Immunol Immunother 62955-965, 2013). One of the major advantages of mass cytometry is the ability to measure multiple intracellular markers, including phosphorylated proteins that are part of major signaling pathways, such as NF-κB, JAK/STAT, and ERK/MAPK. Here we describe an optimized mass cytometry protocol for staining human clinical blood samples with panels that include phosphorylated antibodies.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphoproteins
/
Mass Spectrometry
/
Staining and Labeling
/
Biomarkers
/
Single-Cell Analysis
/
Flow Cytometry
Type of study:
Guideline
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Document type:
Article
Affiliation country:
Australia