LINC01308 accelerates the malignant progression of ovarian cancer by binding to miRNA-506.
Eur Rev Med Pharmacol Sci
; 23(8): 3253-3260, 2019 Apr.
Article
in En
| MEDLINE
| ID: mdl-31081077
ABSTRACT
OBJECTIVE:
To detect the expression pattern of LINC01308 in ovarian cancer (OC), and further clarify whether LINC01308 could promote the malignant progression of OC by mediating microRNA-506 (miRNA-506). PATIENTS ANDMETHODS:
Relative level of LINC01308 in 28 pairs of OC tissues and paracancerous tissues was determined by quantitative Real-time polymerase chain reaction (qRT-PCR). We analyzed the correlation between LINC01308 level and the prognosis of OC patients. Subsequently, LINC01308 level in OC cell lines was determined as well. By transfection of sh-LINC01308 in 3AO and CAOV3 cell lines, we evaluated the influence of LINC01308 on cellular behaviors of OC through cell counting kit-8 (CCK-8), transwell and wound healing assay. Target downstream of LINC01308 was verified by dual-luciferase reporter gene assay. Finally, the regulatory effect of LINC01308/miRNA-506 on OC cell behaviors was examined through a series of rescue experiments.RESULTS:
LINC01308 was highly expressed in OC tissues relative to controls. OC patients with high-level LINC01308 had higher rates of lymph node metastasis and distant metastasis, and lower survival rate compared with those with low level. By transfection of sh-LINC01308 in OC cells, the migratory and invasive abilities were markedly weakened. MiRNA-506 was found to be the target gene of LINC01308, and its level was negatively regulated by LINC01308 in OC tissues. Finally, we found that miRNA-506 knockdown reversed the regulatory effect of LINC01308 on the migratory and invasive abilities of OC cells.CONCLUSIONS:
LINC01308 is highly expressed in OC and correlated to metastasis and poor prognosis. LINC01308 enhances OC cells to migrate and invade by targeting miRNA-506.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Ovarian Neoplasms
/
Cell Movement
/
MicroRNAs
/
Cell Proliferation
/
RNA, Long Noncoding
Limits:
Female
/
Humans
Language:
En
Journal:
Eur Rev Med Pharmacol Sci
Journal subject:
FARMACOLOGIA
/
TOXICOLOGIA
Year:
2019
Document type:
Article
Affiliation country:
China