High-intensity interval training followed by postexercise cold-water immersion does not alter angiogenic circulating cells, but increases circulating endothelial cells.
Appl Physiol Nutr Metab
; 45(1): 101-111, 2020 Jan.
Article
in En
| MEDLINE
| ID: mdl-31167081
ABSTRACT
High-intensity interval training (HIIT) induces vascular adaptations that might be attenuated by postexercise cold-water immersion (CWI). Circulating angiogenic cells (CAC) participate in the vascular adaptations and circulating endothelial cells (CEC) indicate endothelial damage. CAC and CEC are involved in vascular adaptation. Therefore, the aim of the study was to investigate postexercise CWI during HIIT on CAC and CEC and on muscle angiogenesis-related molecules. Seventeen male subjects performed 13 HIIT sessions followed by 15 min of passive recovery (n = 9) or CWI at 10 °C (n = 8). HIIT comprised cycling (8-12 bouts, 90%-110% peak power). The first and the thirteenth sessions were similar (8 bouts at 90% of peak power). Venous blood was drawn before exercise (baseline) and after the recovery strategy (postrecovery) in the first (pretraining) and in the thirteenth (post-training) sessions. For CAC and CEC identification lymphocyte surface markers (CD133, CD34, and VEGFR2) were used. Vastus lateralis muscle biopsies were performed pre- and post-training for protein (p-eNOSser1177) and gene (VEGF and HIF-1) expression analysis related to angiogenesis. CAC was not affected by HIIT or postexercise CWI. Postexercise CWI increased acute and baseline CEC number. Angiogenic protein and genes were not differently modulated by post-CWI. HIIT followed by either recovery strategy did not alter CAC number. Postexercise CWI increased a marker of endothelial damage both acutely and chronically, suggesting that this postexercise recovery strategy might cause endothelial damage. Novelty HIIT followed by CWI did not alter CAC. HIIT followed by CWI increased CEC. Postexercise CWI might cause endothelial damage.
Key words
HIIT; adaptation vasculaire; angiogenic circulating cells; cellules circulantes angiogéniques; cellules endothéliales en circulation; cellules progénitrices endothéliales; circulating endothelial cells; cold-water immersion; cryotherapy; cryothérapie; endothelial progenitor cells; immersion en eau froide; immersion postexercice en eau froide; lésions vasculaires; postexercise cold-water immersion; postexercise recovery; récupération postexercice; vascular adaptation; vascular damage
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood Cells
/
Cold Temperature
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Endothelial Cells
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High-Intensity Interval Training
/
Immersion
Limits:
Adult
/
Humans
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Male
Language:
En
Journal:
Appl Physiol Nutr Metab
Journal subject:
CIENCIAS DA NUTRICAO
/
FISIOLOGIA
/
MEDICINA ESPORTIVA
/
METABOLISMO
Year:
2020
Document type:
Article
Affiliation country:
Brasil