Optimizing the Conditions and Use of Synthetic Matrix for Three-Dimensional In Vitro Retinal Differentiation from Mouse Pluripotent Cells.
Tissue Eng Part C Methods
; 25(7): 433-445, 2019 07.
Article
in En
| MEDLINE
| ID: mdl-31195897
ABSTRACT
IMPACT STATEMENT The development of retinal regenerative therapies relies on the reproducible and renewable source of retinal neurons for drug discovery and cell transplantation. Three-dimensional approach for retinal differentiation from pluripotent cells recently emerged as the robust strategy for retinal tissue differentiation. In this work, we present the combination of optimized conditions and techniques for three-dimensional retinal differentiation from mouse embryonic cells that improves reproducibility and efficiency of retinal differentiation in organoid cultures. We also show that the retinal induction can be achieved with the synthetic oligopeptide instead of Matrigel that allows to approach xeno-free conditions for cell production.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Retina
/
Cell Differentiation
/
Cell Culture Techniques
/
Pluripotent Stem Cells
Limits:
Animals
Language:
En
Journal:
Tissue Eng Part C Methods
Journal subject:
BIOTECNOLOGIA
/
HISTOLOGIA
Year:
2019
Document type:
Article