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Label-Free Multiphoton Microscopy: The Origin of Fluorophores and Capabilities for Analyzing Biochemical Processes.
Shirshin, E A; Yakimov, B P; Darvin, M E; Omelyanenko, N P; Rodionov, S A; Gurfinkel, Y I; Lademann, J; Fadeev, V V; Priezzhev, A V.
Affiliation
  • Shirshin EA; Lomonosov Moscow State University, Faculty of Physics, Moscow, 119991, Russia. shirshin@lid.phys.msu.ru.
  • Yakimov BP; Institute of Spectroscopy, Russian Academy of Sciences, Troitsk, 108840, Moscow, Russia.
  • Darvin ME; Lomonosov Moscow State University, Faculty of Physics, Moscow, 119991, Russia.
  • Omelyanenko NP; Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, 10117, Germany.
  • Rodionov SA; N. N. Priorov National Medical Research Center of Traumatology and Orthopaedics, Moscow, 127299, Russia.
  • Gurfinkel YI; N. N. Priorov National Medical Research Center of Traumatology and Orthopaedics, Moscow, 127299, Russia.
  • Lademann J; Medical Scientific-Educational Center of Lomonosov Moscow State University, Moscow, 119192, Russia.
  • Fadeev VV; Center of Experimental and Applied Cutaneous Physiology, Department of Dermatology, Venerology and Allergology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Berlin, 10117, Germany.
  • Priezzhev AV; Lomonosov Moscow State University, Faculty of Physics, Moscow, 119991, Russia.
Biochemistry (Mosc) ; 84(Suppl 1): S69-S88, 2019 Jan.
Article in En | MEDLINE | ID: mdl-31213196
ABSTRACT
Multiphoton microscopy (MPM) is a method of molecular imaging and specifically of intravital imaging that is characterized by high spatial resolution in combination with a greater depth of penetration into the tissue. MPM is a multimodal method based on detection of nonlinear optical signals - multiphoton fluorescence and optical harmonics - and also allows imaging with the use of the parameters of fluorescence decay kinetics. This review describes and discusses photophysical processes within major reporter molecules used in MPM with endogenous contrasts and summarizes several modern experiments that illustrate the capabilities of label-free MPM for molecular imaging of biochemical processes in connective tissue and cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biochemical Phenomena / Cells / Connective Tissue / Microscopy, Fluorescence, Multiphoton / Optical Imaging / Fluorescent Dyes Limits: Humans Language: En Journal: Biochemistry (Mosc) Year: 2019 Document type: Article Affiliation country: Rusia
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biochemical Phenomena / Cells / Connective Tissue / Microscopy, Fluorescence, Multiphoton / Optical Imaging / Fluorescent Dyes Limits: Humans Language: En Journal: Biochemistry (Mosc) Year: 2019 Document type: Article Affiliation country: Rusia