Next-generation protein analysis in the pathology department.
J Clin Pathol
; 73(1): 1-6, 2020 Jan.
Article
in En
| MEDLINE
| ID: mdl-31308255
ABSTRACT
Traditionally, immunohistochemistry (IHC) is used by pathologists to localise specific proteins or peptides in tissue slides. In the era of personalised medicine, however, molecular tissue analysis becomes indispensable for correct diagnosis, prognosis and therapeutic decision, not only on the DNA or mRNA level but also on the protein level. Combining molecular information with imaging presents many advantages. Therefore, matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI IMS) is a promising technique to be added to the armamentarium of the pathologist. Here, we focus on the workflow, advantages and drawbacks of both MALDI IMS and IHC. We also briefly discuss a few other protein imaging modalities and give examples of applications.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Pathology, Clinical
/
Pathology Department, Hospital
/
Immunohistochemistry
/
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
/
Proteomics
/
High-Throughput Screening Assays
Type of study:
Prognostic_studies
/
Sysrev_observational_studies
Limits:
Humans
Language:
En
Journal:
J Clin Pathol
Year:
2020
Document type:
Article
Affiliation country:
Bélgica