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Microbiota-derived acetate protects against respiratory syncytial virus infection through a GPR43-type 1 interferon response.
Antunes, Krist Helen; Fachi, José Luís; de Paula, Rosemeire; da Silva, Emanuelle Fraga; Pral, Laís Passariello; Dos Santos, Adara Áurea; Dias, Greicy Brisa Malaquias; Vargas, José Eduardo; Puga, Renato; Mayer, Fabiana Quoos; Maito, Fábio; Zárate-Bladés, Carlos R; Ajami, Nadim J; Sant'Ana, Marcella Ramos; Candreva, Thamiris; Rodrigues, Hosana Gomes; Schmiele, Marcio; Silva Clerici, Maria Teresa Pedrosa; Proença-Modena, José Luiz; Vieira, Angélica Thomas; Mackay, Charles R; Mansur, Daniel; Caballero, Mauricio T; Marzec, Jacqui; Li, Jianying; Wang, Xuting; Bell, Douglas; Polack, Fernando P; Kleeberger, Steven R; Stein, Renato T; Vinolo, Marco Aurélio Ramirez; de Souza, Ana Paula Duarte.
Affiliation
  • Antunes KH; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, 90610-000, RS, Brazil.
  • Fachi JL; Laboratory of Immunoinflammation, Department of Genetics, Evolution, Microbiology and Immunology - Institute of Biology, University of Campinas, Campinas, 13083007, São Paulo, Brazil.
  • de Paula R; Laboratory of Immunoinflammation, Department of Genetics, Evolution, Microbiology and Immunology - Institute of Biology, University of Campinas, Campinas, 13083007, São Paulo, Brazil.
  • da Silva EF; Laboratory of Clinical and Experimental Immunology, Infant Center, School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, 90610-000, RS, Brazil.
  • Pral LP; Laboratory of Immunoinflammation, Department of Genetics, Evolution, Microbiology and Immunology - Institute of Biology, University of Campinas, Campinas, 13083007, São Paulo, Brazil.
  • Dos Santos AÁ; Laboratory of Imunobiology, Departament of Microbiology, Immunology and Parasitology, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Santa Catarina, 88040900, Brazil.
  • Dias GBM; Laboratory of Imunobiology, Departament of Microbiology, Immunology and Parasitology, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Santa Catarina, 88040900, Brazil.
  • Vargas JE; Biological Science Institute (ICB), Passo Fundo University, Passo Fundo, 99052900, State of Rio Grande do Sul, Brazil.
  • Puga R; Clinical Research Center, Hospital Israelita Albert Einstein HIAE, São Paulo, 05652900, Brazil.
  • Mayer FQ; Molecular Biology Laboratory, Veterinary Research Institute Desidério Finamor, Agricultural Diagnosis and Research Department, Secretariat of Agriculture, Livestock and Irrigation, Eldorado do Sul, 92990000, RS, Brazil.
  • Maito F; Laboratory of Pathology, Healthy Science School, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, 90610-000, RS, Brazil.
  • Zárate-Bladés CR; Laboratory of Iimmunoregulation, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, UFSC, Florianopolis, 8804900, SC, Brazil.
  • Ajami NJ; Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, 77030, USA.
  • Sant'Ana MR; Laboratory of Nutritional Genomics, School of Applied Sciences, University of Campinas, Limeira, 13484350, São Paulo, Brazil.
  • Candreva T; Laboratory of Nutrients and Tissue Repair, School of Applied Sciences, University of Campinas, Limeira, 13484350, São Paulo, Brazil.
  • Rodrigues HG; Laboratory of Nutrients and Tissue Repair, School of Applied Sciences, University of Campinas, Limeira, 13484350, São Paulo, Brazil.
  • Schmiele M; Institute of Science and Technology, Federal University of Jequitinhonha and Mucuri Valleys (UFVJM), Teófilo Otoni, 39803371, MG, Brazil.
  • Silva Clerici MTP; Department of Food Technology, School of Food Engineering, University of Campinas (UNICAMP) - Cidade Universitária Zeferino Vaz, Monteiro Lobato, 80, Campinas, 13083970, São Paulo, Brazil.
  • Proença-Modena JL; Emerging viruses study Laboratory, Department of Genetics, Evolution, Microbiology and Immunology, Institute of Biology, University of Campinas, Campinas, 13083970, Brazil.
  • Vieira AT; Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, 31270901, MG, Brazil.
  • Mackay CR; Biodiscovery Research Institute, Monash University, Clayton, 3800, Australia.
  • Mansur D; Laboratory of Imunobiology, Departament of Microbiology, Immunology and Parasitology, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Santa Catarina, 88040900, Brazil.
  • Caballero MT; Fundación INFANT, Buenos Aires, 1406, Argentina.
  • Marzec J; National Institute of Environmental Health Sciences (NIEHS), NIH, Research Triangle, Durham, 27709, NC, USA.
  • Li J; National Institute of Environmental Health Sciences (NIEHS), NIH, Research Triangle, Durham, 27709, NC, USA.
  • Wang X; National Institute of Environmental Health Sciences (NIEHS), NIH, Research Triangle, Durham, 27709, NC, USA.
  • Bell D; National Institute of Environmental Health Sciences (NIEHS), NIH, Research Triangle, Durham, 27709, NC, USA.
  • Polack FP; Fundación INFANT, Buenos Aires, 1406, Argentina.
  • Kleeberger SR; Vanderbilt University, EUA, Nashville, 37240, TN, USA.
  • Stein RT; National Institute of Environmental Health Sciences (NIEHS), NIH, Research Triangle, Durham, 27709, NC, USA.
  • Vinolo MAR; Infant Center, School of Medicine, Department of Pediatrics, São Lucas Hospital PUCRS, Porto Alegre, 90610-000, RS, Brazil.
  • de Souza APD; Laboratory of Immunoinflammation, Department of Genetics, Evolution, Microbiology and Immunology - Institute of Biology, University of Campinas, Campinas, 13083007, São Paulo, Brazil. mvinolo@unicamp.br.
Nat Commun ; 10(1): 3273, 2019 07 22.
Article in En | MEDLINE | ID: mdl-31332169
Severe respiratory syncytial virus (RSV) infection is a major cause of morbidity and mortality in infants <2 years-old. Here we describe that high-fiber diet protects mice from RSV infection. This effect was dependent on intestinal microbiota and production of acetate. Oral administration of acetate mediated interferon-ß (IFN-ß) response by increasing expression of interferon-stimulated genes in the lung. These effects were associated with reduction of viral load and pulmonary inflammation in RSV-infected mice. Type 1 IFN signaling via the IFN-1 receptor (IFNAR) was essential for acetate antiviral activity in pulmonary epithelial cell lines and for the acetate protective effect in RSV-infected mice. Activation of Gpr43 in pulmonary epithelial cells reduced virus-induced cytotoxicity and promoted antiviral effects through IFN-ß response. The effect of acetate on RSV infection was abolished in Gpr43-/- mice. Our findings reveal antiviral effects of acetate involving IFN-ß in lung epithelial cells and engagement of GPR43 and IFNAR.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Interferon Type I / Respiratory Syncytial Virus Infections / Receptors, G-Protein-Coupled / Microbiota / Acetates Limits: Animals / Humans Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2019 Document type: Article Affiliation country: Brasil Country of publication: Reino Unido
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Interferon Type I / Respiratory Syncytial Virus Infections / Receptors, G-Protein-Coupled / Microbiota / Acetates Limits: Animals / Humans Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2019 Document type: Article Affiliation country: Brasil Country of publication: Reino Unido