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A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs.
Macori, Guerrino; McCarthy, Siobhán C; Burgess, Catherine M; Fanning, Séamus; Duffy, Geraldine.
Affiliation
  • Macori G; Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.
  • McCarthy SC; Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland; UCD-Centre for Food Safety, School of Public Health, Physiotherapy & Sports Science, University College Dublin, Belfield, Dublin D04 N2E5, Ireland.
  • Burgess CM; Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland. Electronic address: kaye.burgess@teagasc.ie.
  • Fanning S; UCD-Centre for Food Safety, School of Public Health, Physiotherapy & Sports Science, University College Dublin, Belfield, Dublin D04 N2E5, Ireland.
  • Duffy G; Teagasc Food Research Centre, Ashtown, Dublin 15, Ireland.
J Microbiol Methods ; 165: 105703, 2019 10.
Article in En | MEDLINE | ID: mdl-31454506
ABSTRACT
A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values to the initial concentration of pathogen in the sheep sample.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep / Colony Count, Microbial / Escherichia coli O157 / Bacterial Shedding / Real-Time Polymerase Chain Reaction Limits: Animals Language: En Journal: J Microbiol Methods Year: 2019 Document type: Article Affiliation country: Irlanda
Fulltext
Add to My VHL
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PubMed Links
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Sheep / Colony Count, Microbial / Escherichia coli O157 / Bacterial Shedding / Real-Time Polymerase Chain Reaction Limits: Animals Language: En Journal: J Microbiol Methods Year: 2019 Document type: Article Affiliation country: Irlanda