SERS-based particle tracking and molecular imaging in live cells: toward the monitoring of intracellular dynamics.
Nanoscale
; 11(45): 21724-21727, 2019 Nov 21.
Article
in En
| MEDLINE
| ID: mdl-31495836
ABSTRACT
Although diverse endogenous biomolecules involved in life processes are of major interest in cell biology, there is still a lack of suitable methods for studying biomolecules within live cells without labelling. Herein, we describe a near-infrared (NIR) surface-enhanced Raman scattering (SERS)-based particle tracking technique gathering chemical information inside live cells for monitoring their intracellular dynamics. The wide-field SERS imaging spectroscopy system facilitates high temporal resolution (200 ms) under high spatial resolution (512 × 512 pixels) for one live cell. With high spatiotemporal resolution and signal-to-background ratio, we show that the Raman signal from intracellular cargoes in live cells is sporadically observed and localized to a vesicular level.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cytoplasm
/
Metal Nanoparticles
/
Molecular Imaging
/
Gold
Limits:
Humans
Language:
En
Journal:
Nanoscale
Year:
2019
Document type:
Article
Affiliation country:
Corea del Sur