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Placental production of progestins is fully effective in villous cytotrophoblasts and increases with the syncytiotrophoblast formation.
Fraichard, C; Bonnet, F; Garnier, A; Hébert-Schuster, M; Bouzerara, A; Gerbaud, P; Ferecatu, I; Fournier, T; Hernandez, I; Trabado, S; Guibourdenche, J.
Affiliation
  • Fraichard C; UMR-S 1139, Faculté de Pharmacie, Université Paris Descartes, Paris, France.
  • Bonnet F; Service d' Hormonologie, CHU Cochin, HUPC, AP-HP, Paris, France.
  • Garnier A; Service de Génétique Moléculaire, Pharmacogénétique et Hormonologie, Hôpitaux Universitaires Paris-Sud, CHU de Bicêtre, AP-HP, Le Kremlin Bicêtre, France; UMR-S U1185, Faculté de Médecine Paris-Sud, Université Paris-Sud, Université Paris-Saclay, Le Kremlin Bicêtre, France.
  • Hébert-Schuster M; UMR-S 1139, Faculté de Pharmacie, Université Paris Descartes, Paris, France; Service d' Hormonologie, CHU Cochin, HUPC, AP-HP, Paris, France.
  • Bouzerara A; UMR-S 1139, Faculté de Pharmacie, Université Paris Descartes, Paris, France; Service d' Hormonologie, CHU Cochin, HUPC, AP-HP, Paris, France.
  • Gerbaud P; UMR-S 1180, Faculté de Pharmacie, 5 Rue JB Clément, 92296, Châtenay-Malabry, France.
  • Ferecatu I; UMR-S 1139, Faculté de Pharmacie, Université Paris Descartes, Paris, France.
  • Fournier T; UMR-S 1139, Faculté de Pharmacie, Université Paris Descartes, Paris, France.
  • Hernandez I; UMR-S 1139, Faculté de Pharmacie, Université Paris Descartes, Paris, France.
  • Trabado S; Service de Génétique Moléculaire, Pharmacogénétique et Hormonologie, Hôpitaux Universitaires Paris-Sud, CHU de Bicêtre, AP-HP, Le Kremlin Bicêtre, France; UMR-S U1185, Faculté de Médecine Paris-Sud, Université Paris-Sud, Université Paris-Saclay, Le Kremlin Bicêtre, France.
  • Guibourdenche J; UMR-S 1139, Faculté de Pharmacie, Université Paris Descartes, Paris, France; Service d' Hormonologie, CHU Cochin, HUPC, AP-HP, Paris, France. Electronic address: jean.guibourdenche@aphp.fr.
Mol Cell Endocrinol ; 499: 110586, 2020 01 01.
Article in En | MEDLINE | ID: mdl-31539598
ABSTRACT
Placental syncytiotrophoblast (ST) is considered as the main placental endocrine tissue secreting progesterone, a steroid essential for maintenance of pregnancy. However, each step of progestins production has been poorly investigated in villous cytotrophoblast (VCT) regarding ST formation. We aimed to characterize progestins production during human differentiation of VCT into ST. VCTs were isolated from term placenta and cultivated, with or without forskolin (FSK), to stimulate trophoblast differentiation. Secreted progestins concentrations were determined by immuno-assay and Gas Chromatography-tandem mass spectrometry. Intracellular expression of cholesterol transporter and enzymes involved in steroidogenesis were studied by immunofluorescence, western-blot, and RT-qPCR. Progesterone and pregnenolone are produced by VCT and their secretion increases with VCT differentiation while 17-hydroxyprogesterone concentration remains undetectable. HSD3B1 enzyme expression increases whereas MLN64, the cholesterol placental mitochondrial transporter and P450SCC expressions do not. FSK induces progestins production. Progestins placental synthesis is effective since VCT and increases with ST formation thanks to mitochondria.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Placenta / Progestins / Progesterone Reductase / Steroid Isomerases / Trophoblasts / TNF Receptor-Associated Factor 4 / Multienzyme Complexes Limits: Female / Humans / Pregnancy Language: En Journal: Mol Cell Endocrinol Year: 2020 Document type: Article Affiliation country: Francia
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Placenta / Progestins / Progesterone Reductase / Steroid Isomerases / Trophoblasts / TNF Receptor-Associated Factor 4 / Multienzyme Complexes Limits: Female / Humans / Pregnancy Language: En Journal: Mol Cell Endocrinol Year: 2020 Document type: Article Affiliation country: Francia