Thermodynamic and Structural Behavior of α-Galactosylceramide and C6-Functionalized α-GalCer in 2D Layers at the Air-Liquid Interface.
Chembiochem
; 21(1-2): 241-247, 2020 01 15.
Article
in En
| MEDLINE
| ID: mdl-31544285
ABSTRACT
α-Galactosylceramide (α-GalCer; KRN7000) is a ligand for the glycoprotein CD1d that presents lipid antigens to natural killer T cells. Therefore, KRN7000 as well as some modified versions thereof have been widely investigated as part of novel immunotherapies. To examine the impact of structural modification, we investigated KRN7000 and C6-modified KRN7000 at the air-liquid interface using monolayer isotherms, BAM, IRRAS, GIXD, and TRXF. The amino group has no influence on the highly ordered sub-gel structures found at lateral pressures relevant for biological membranes. Neither lateral compression nor the protonation state of the amino group has a measurable effect on the lattice structure, which is defined by strong and rigid intermolecular hydrogen bonds. However, the first-order phase transition found for the C6-functionalized α-GalCer is connected with an extraordinary surface-inhibited nucleation. Our study demonstrates that KRN7000 can be functionalized at C6 without significantly changing the structural properties.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thermodynamics
/
Galactosylceramides
/
Nitrogen
Language:
En
Journal:
Chembiochem
Journal subject:
BIOQUIMICA
Year:
2020
Document type:
Article
Affiliation country:
Alemania