Albumin-chaperoned cyanine dye yields superbright NIR-II fluorophore with enhanced pharmacokinetics.
Sci Adv
; 5(9): eaaw0672, 2019 09.
Article
in En
| MEDLINE
| ID: mdl-31548981
ABSTRACT
NIR-II fluorescence imaging greatly reduces scattering coefficients for nearly all tissue types at long wavelengths, benefiting deep tissue imaging. However, most of the NIR-II fluorophores suffer from low quantum yields and/or short circulation time that limit the quality of NIR-II imaging. Here, we engineered a supramolecular assembly of protein complex with lodged cyanine dyes to produce a brilliant NIR-II fluorophore, providing a NIR-II quantum yield of 21.2% with prolonged circulation time. Computational modeling revealed the mechanism for fluorescence enhancement and identified key parameters governing albumin complex for NIR-II fluorophores. Our complex afforded high-resolution microvessel imaging, with a 3-hour imaging window compared to 2 min for free dye alone. Furthermore, the complexation strategy was applied to an antibody-derived assembly, offering high-contrast tumor imaging without affecting the targeting ability of the antibody. This study provides a facile strategy for producing high-performance NIR-II fluorophores by chaperoning cyanine dyes with functional proteins.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Serum Albumin, Bovine
/
Contrast Media
/
Optical Imaging
/
Fluorescent Dyes
/
Neoplasms, Experimental
Limits:
Animals
Language:
En
Journal:
Sci Adv
Year:
2019
Document type:
Article
Affiliation country:
Estados Unidos