Optimizing the safety of intradiscal platelet-rich plasma: an in vitro study with Cutibacterium acnes.
Regen Med
; 14(10): 955-967, 2019 10.
Article
in En
| MEDLINE
| ID: mdl-31587600
ABSTRACT
Aim:
The most common risk associated with intradiscal injection of platelet-rich plasma (PRP) is discitis with Cutibacterium acnes. It is hypothesized that antimicrobial activity of PRP can be enhanced through inclusion of leukocytes or antibiotics in the injectate. Materials &methods:
Multiple PRP preparations of varying platelet and leukocyte counts were co-cultured with C. acnes with or without cefazolin, with viable bacterial colony counts being recovered at 0, 4, 24 and 48 hours post-inoculation.Results:
A direct correlation between C. acnes recovery and granulocyte counts were observed.Conclusion:
We observed the greatest antimicrobial activity with the leukocyte-rich, high platelet PRP preparation combined with an antibiotic in the injectate. However, cefazolin did not completely clear the bacteria in this assay.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Propionibacteriaceae
/
Blood Bactericidal Activity
/
Microbial Viability
/
Platelet-Rich Plasma
Limits:
Female
/
Humans
/
Male
Language:
En
Journal:
Regen Med
Year:
2019
Document type:
Article
Affiliation country:
Estados Unidos