FluChip-8G Insight: HA and NA subtyping of potentially pandemic influenza A viruses in a single assay.
Influenza Other Respir Viruses
; 14(1): 55-60, 2020 01.
Article
in En
| MEDLINE
| ID: mdl-31608599
ABSTRACT
BACKGROUND:
Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip-8G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip-8G Insight uses whole gene segment RT-PCR-based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network-based data interpretation.OBJECTIVES:
The objective of this study was to verify and validate the performance of the FluChip-8G Insight assay for the detection and positive identification of human and animal origin non-seasonal influenza A specimens.METHODS:
We evaluated the ability of the FluChip-8G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non-seasonal influenza A strains (49 unique subtypes).RESULTS:
FluChip-8G Insight demonstrated a positive percent agreement ≥93% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes.CONCLUSIONS:
The FluChip-8G Insight neural network-based algorithm used for virus identification performed well over a data set of 297 naïve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Influenza A virus
/
Viral Proteins
/
Hemagglutinin Glycoproteins, Influenza Virus
/
Reverse Transcriptase Polymerase Chain Reaction
/
Influenza, Human
/
Neuraminidase
Type of study:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limits:
Humans
Country/Region as subject:
America do norte
Language:
En
Journal:
Influenza Other Respir Viruses
Journal subject:
VIROLOGIA
Year:
2020
Document type:
Article
Affiliation country:
Estados Unidos