Exploiting nanobodies and Affimers for superresolution imaging in light microscopy.
Mol Biol Cell
; 30(22): 2737-2740, 2019 10 15.
Article
in En
| MEDLINE
| ID: mdl-31609674
ABSTRACT
Antibodies have long been the main approach used for localizing proteins of interest by light microscopy. In the past 5 yr or so, and with the advent of superresolution microscopy, the diversity of tools for imaging has rapidly expanded. One main area of expansion has been in the area of nanobodies, small single-chain antibodies from camelids or sharks. The other has been the use of artificial scaffold proteins, including Affimers. The small size of nanobodies and Affimers compared with the traditional antibody provides several advantages for superresolution imaging.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Single-Domain Antibodies
/
Microscopy
Limits:
Animals
/
Humans
Language:
En
Journal:
Mol Biol Cell
Journal subject:
BIOLOGIA MOLECULAR
Year:
2019
Document type:
Article
Affiliation country:
Reino Unido