Proteoglycan/glycosaminoglycan and collagen content in the arterial wall of patients with end-stage renal disease: new indicators of vascular disease.
Pol Arch Intern Med
; 129(11): 781-789, 2019 11 29.
Article
in En
| MEDLINE
| ID: mdl-31613870
ABSTRACT
INTRODUCTION:
The prevalence of cardiovascular (CV) comorbidity in patients with chronic kidney disease (CKD) is high, particularly in endstage renal disease (ESRD). There is an ongoing search for novel biomarkers of CV disease in this population.OBJECTIVES:
We aimed to investigate the associations of matrix proteoglycans (PGs) and glycosaminoglycans (GAGs), collagen, and arterial calcifications with selected serum and plasma markers of endothelial dysfunction, inflammation, oxidative stress, and bone turnover in patients with ESRD. PATIENTS ANDMETHODS:
We enrolled 47 adult patients (32 men) with stage 5 CKD. The following parameters were investigated fibrinogen, soluble thrombomodulin (sTM), plasminogen activator inhibitor 1 (PAI1), stromal cellderived factor 1α (SDF1α), calcium (Ca), phosphate (Pi), intact parathormone, interleukin 6, highsensitivity Creactive protein (hsCRP), ferric reducing ability of plasma, 2,2diphenyl1picrylhydrazyl scavenging, ferric reducing ability of ascorbate in plasma, fetuinA, fibroblast growth factor 23, osteopontin, osteoprotegerin, osteocalcin, transforming growth factor ß (TGFß), hepatocyte growth factor, secreted protein acidic and rich in cysteine, as well as matrix metalloproteinase 2. Radial artery specimens were stained with alizarin red for calcifications, alcian blue for PGs and GAGs, and sirius red for collagen.RESULTS:
We observed positive correlations between PG and GAG, collagen, and calcification staining. The most intense (grade 3) alcian blue staining was significantly correlated with diabetes as well as higher levels of Ca × Pi product, hsCRP, fibrinogen, SDF1α, PAI1, and sTM. However, PAI1 was the only significant predictor of grade 3 alcian blue staining in a multiple logistic regression model adjusted for hemodialysis, Ca× Pi product, and hsCRP levels.CONCLUSIONS:
Coagulation disorders and endothelial dysfunction are the hallmarks of ESRD. The levels of SDF1α, PAI1, sTM, and fibrinogen may be novel predictors of early vascular wall alterations and may serve as CV risk markers.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Proteoglycans
/
Biomarkers
/
Cardiovascular Diseases
/
Collagen
/
Radial Artery
/
Glycosaminoglycans
/
Kidney Failure, Chronic
Type of study:
Prognostic_studies
/
Risk_factors_studies
Limits:
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Country/Region as subject:
Europa
Language:
En
Journal:
Pol Arch Intern Med
Year:
2019
Document type:
Article
Affiliation country:
Polonia