Effects of resveratrol on intestinal oxidative status and inflammation in heat-stressed rats.

ABSTRACT

Heat stress, experienced by humans and animals under high ambient temperatures, is known to induce oxidative stress and inflammation, which endangers human health as well as animal welfare and production. The gastrointestinal tract is predominantly responsive to heat stress and compromised intestinal functions can contribute to multi-organ injury under heat environment. Resveratrol (RSV) has significant antioxidant and anti-inflammatory activities. The aim of this study was to investigate the potential effects of RSV on intestinal function (digestion and barrier), oxidative stress and inflammation in heat-stressed rats. Male Sprague-Dawley rats were orally fed with 100 mg RSV/kg body weight/day prior to daily heat stress (40 °C per day for 1.5 h) exposure for 3 consecutive days. The results showed that RSV reversed the increased serum cortisol level and diamine oxidase activity, the altered jejunal morphology, the decreased jejunal disaccharidase activities, the elevated malondialdehyde and tumor necrosis factor alpha concentrations and antioxidant enzymes activities in the jejunum, as well as the increased jejunal mRNA expression of toll-like receptor 4, cytokines, antioxidant enzymes and tight junction proteins in heat-stressed rats, to various degrees. In conclusion, RSV could alleviate intestinal injury and dysfunctions by improving oxidative status and suppressing inflammation in heat-stressed rats.