Review of functional in vitro models of the blood-cerebrospinal fluid barrier in leukaemia research.
J Neurosci Methods
; 329: 108478, 2020 01 01.
Article
in En
| MEDLINE
| ID: mdl-31669338
ABSTRACT
Acute lymphoblastic leukaemia represents the most common paediatric malignancy. Although survival rates approach up to 90% in children, investigation of leukaemic infiltration into the central nervous system (CNS) is essential due to the presence of ongoing fatal complications. Recent in vitro studies mostly employed models of the blood-brain barrier (BBB), as endothelial cells of the microvasculature represent the largest surface between the blood stream and the brain parenchyma. However, crossing the blood-cerebrospinal fluid barrier (BCSFB) within the choroid plexus (CP) has been shown to be a general capability of leukaemic blasts. Hence, in vitro models of the BCSFB to study leukaemic transmigration may be of major importance to understand the development of CNS leukaemia. This review will summarise available in vitro models of the BCSFB employed to study the cellular interactions with leukaemic blasts during cancer cell transmigration into the brain compartment across primary or immortal/immortalised BCSFB cells. It will also provide an outlook on prospective improvements in BCSFB in vitro models by developing barrier-on-a-chip models and brain organoids.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blood-Brain Barrier
/
Cerebrospinal Fluid
/
Choroid Plexus
/
Cell Line, Tumor
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Transcellular Cell Migration
/
Primary Cell Culture
Limits:
Animals
/
Humans
Language:
En
Journal:
J Neurosci Methods
Year:
2020
Document type:
Article